產(chǎn)品[
Hs 738.St/Int 人成纖維細(xì)胞
]資料
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產(chǎn)品名稱:
Hs 738.St/Int 人成纖維細(xì)胞
產(chǎn)品型號(hào):
CRL-7869
產(chǎn)品廠商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無相關(guān)文檔
簡(jiǎn)單介紹
CRL-7869 Hs 738.St/Int 人成纖維細(xì)胞，原代細(xì)胞|細(xì)胞系|細(xì)胞株|腫瘤細(xì)胞；細(xì)胞庫管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和培養(yǎng)條件！
Hs 738.St/Int 人成纖維細(xì)胞
的詳細(xì)介紹
CRL-7869 Hs 738.St/Int 人成纖維細(xì)胞
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CRL-7869?
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Hs 738.St/Int
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Biosafety Level:
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1
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frozen
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Medium & Serum:
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See Propagation
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adherent
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Homo sapiens (human)CRL-7869 Hs 738.St/Int 人成纖維細(xì)胞
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fibroblast
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Organ: mixed; stomach; intestine
Disease: normal
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8,11
D16S539: 9,11
D5S818: 11,13
D7S820: 8,12
THO1: 7,9.3
TPOX: 8
vWA: 16,18
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modal number = 46; range = 44 to 92
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18 weeks gestation fetus
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CRL-7869 Hs 738.St/Int 人成纖維細(xì)胞male
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
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Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended
Medium Renewal: Every 2 to 3 days
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Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor termperature
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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CRL-7869 Hs 738.St/Int 人成纖維細(xì)胞
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