產品資料
產品[

CRL-1993 HMy2.CIR 人B**母細胞

]資料
如果您對該產品感興趣的話,可以 sendmsg
產品名稱: CRL-1993 HMy2.CIR 人B**母細胞
產品型號: CRL-1993
產品廠商: 美國標準生物品收藏中心(ATCC)
產品文檔: 無相關文檔


簡單介紹

CRL-1993 HMy2.CIR 人B**母細胞,ATCC 細胞|細胞系|細胞株|腫瘤細胞|細胞|貼壁細胞|懸浮細胞|;細胞庫管理規(guī)范,提供的細胞株背景清楚,提供參考文獻和Z優(yōu)培養(yǎng)條件!


CRL-1993 HMy2.CIR 人B**母細胞 的詳細介紹
CRL-1993 HMy2.CIR 人B**母細胞
ATCC® Number: CRL-1993?     
Designations: HMy2.CIR [C1R, HMy2.C1R]
Depositors:  P Cresswell
Biosafety Level: 2 [Cells contain Eptein-Barr Viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast

Source: Cell Type: B lymphoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
DNA Profile (STR): Amelogenin: X
CSF1PO: 6,10
D13S317: 11,13
D16S539: 9,13
D5S818: 10,13
D7S820: 7,12
THO1: 8
TPOX: 8
vWA: 17
Comments: The CRL-1621cell line is reported to be positive for Epstein-Barr nuclear antigen (EBNA +) and Epstein-Barr viral capsid antigen (EBVCA +). Since the HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621), it is assumed to be EBNA positive.
The HMy2.CIR cell line was derived from the HMy.2 B lymphoblastoid cell line, a fast growing mutant of the ARH-77 cell line (ATCC CRL-1621), by gamma irradiation and selection for loss of HLA class I antigen expression. [23312]
The cells express no HLA A or B locus products, but do express small amounts of HLA Cw4.
The cells are useful as host for transfected class I major histocompatibility antigen genes.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) viable cells/ml.
Interval: Maintain between 2 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: parental cell line:ATCC CRL-1621
derivative:ATCC CRL-2369
derivative:ATCC CRL-2370
derivative:ATCC CRL-2371
References: 23312: Storkus WJ, et al. Reversal of natural killing susceptibility in target cells expressing transfected class I HLA genes. Proc. Natl. Acad. Sci. USA 86: 2361-2364, 1989. PubMed: 2784569
產品留言
標題
聯(lián)系人
聯(lián)系電話
內容
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發(fā)送信息!
2.如有必要,請您留下您的詳細聯(lián)系方式!

聯(lián)系我們
產品搜索

細胞系,ATCC細胞,ATCC菌種,細胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細胞庫,人**瘤細胞,人食管癌細胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細胞株,標準菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細胞目錄,質控菌株,腫瘤細胞株,乳腺癌細胞系,人肝癌細胞系

產品目錄

Copyright? 2003-2025  上海復祥生物科技有限公司版權所有            電話:021-35080510
傳真:021-35080520    地址:上海市虹口區(qū)赤峰路630號南雁大廈1901室    郵編: 滬ICP備10013034號-3  

  

滬公網(wǎng)安備 31010902002429號