產(chǎn)品[
CRL-2580 MR1 中國(guó)倉(cāng)鼠X小鼠B**細(xì)胞雜交瘤
]資料
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產(chǎn)品名稱(chēng):
CRL-2580 MR1 中國(guó)倉(cāng)鼠X小鼠B**細(xì)胞雜交瘤
產(chǎn)品型號(hào):
CRL-2580
產(chǎn)品廠(chǎng)商:
美國(guó)標(biāo)準(zhǔn)生物品收藏中心（ATCC）
產(chǎn)品文檔:
無(wú)相關(guān)文檔
簡(jiǎn)單介紹
CRL-2580 MR1 中國(guó)倉(cāng)鼠X小鼠B**細(xì)胞雜交瘤，原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種；細(xì)胞庫(kù)管理規(guī)范，提供的細(xì)胞株背景清楚，提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件！
CRL-2580 MR1 中國(guó)倉(cāng)鼠X小鼠B**細(xì)胞雜交瘤
的詳細(xì)介紹
CRL-2580 MR1 中國(guó)倉(cāng)鼠X小鼠B**細(xì)胞雜交瘤
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CRL-2580?
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$323.00
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MR1 [derivative of HB-11048]
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Bristol-Myers Squibb Pharm. Res. Inst.
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IgG
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Biosafety Level:
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1
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frozen
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See Propagation
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Cricetulus migratorius (B cell); Mus musculus (myeloma) (hamster, Armenian (B cell); mouse (myeloma))
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lymphoblast
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Cell Type: hybridoma: B lymphocyte;
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immunoglobulin; monoclonal antibody; against mouse CD40 ligand (CD154, CD40L, gp39)
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate and supplemented with 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
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Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 to 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.
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Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5%
Storage temperature: liquid nitrogen vapor phase
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC HB-11048
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23334: Noelle RJ, et al. A 39-kDa protein on activated helper T cells binds CD40 and transduces the signal for cognate activation of B cells. Proc. Natl. Acad. Sci. USA 89: 6550-6554, 1992. PubMed: 1378631
23483: Roy M, et al. The regulation of the expression of gp39, the CD40 ligand, on normal and cloned CD4+ T cells. J. Immunol. 151: 2497-2510, 1993. PubMed: 8103067
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