產(chǎn)品資料
產(chǎn)品[

小鼠B細(xì)胞雜交瘤細(xì)胞

]資料
如果您對該產(chǎn)品感興趣的話,可以 sendmsg
產(chǎn)品名稱: 小鼠B細(xì)胞雜交瘤細(xì)胞
產(chǎn)品型號: HB-95
產(chǎn)品廠商: 美國標(biāo)準(zhǔn)生物品收藏中心(ATCC)
產(chǎn)品文檔: 無相關(guān)文檔


簡單介紹

HB-95 W6/32 小鼠B細(xì)胞雜交瘤細(xì)胞,原代細(xì)胞|細(xì)胞系|細(xì)胞株|菌種;細(xì)胞庫管理規(guī)范,提供的細(xì)胞株背景清楚,提供參考文獻(xiàn)和Z優(yōu)培養(yǎng)條件!


小鼠B細(xì)胞雜交瘤細(xì)胞 的詳細(xì)介紹
HB-95 W6/32 小鼠B細(xì)胞雜交瘤細(xì)胞
ATCC® Number: HB-95?    Price: $323.00
Designations: W6/32
Depositors:  P Parham
Isotype: IgG2a
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))
Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against HLA A, B, C
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Comments: Animals were immunized with cells from human tonsils.
Spleen cells were fused with P3X63Ag8 myeloma cells.
The antibody reacts with monomorphic determinants on HLA A,B,C molecules.
The antibody is cytotoxic.
The antibody cross-reacts with ape, monkey and cow cells.
It does not react with rabbit cells.
Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Freeze medium: 95% FBS ; 5% DMSO
Storage temperature: liquid nitrogen vapor phase
References: 955: Brodsky FM, Parham P. Monomorphic anti-HLA-A,B,C monoclonal antibodies detecting molecular subunits and combinatorial determinants. J. Immunol. 128: 129-135, 1982. PubMed: 6172474
956: Barnstable CJ, et al. Production of monoclonal antibodies to group A erythrocytes, HLA and other human cell surface antigens -- new tools for genetic analysis. Cell 14: 9-20, 1978. PubMed: 667938
22830: Parham P, et al. Use of a monoclonal antibody (W6/32) in structural studies of HLA- A,B,C, antigens. J. Immunol. 123: 342-349, 1979. PubMed: 87477
32767: Solomon KR, et al. The association between glycoslphosphatidylinositol-anchored proteins and hetrotrimeric G protein alpha subunits in lymphocytes. Proc. Natl. Acad. Sci. USA 93: 6053-6058, 1996. PubMed: 8650218
產(chǎn)品留言
標(biāo)題
聯(lián)系人
聯(lián)系電話
內(nèi)容
驗證碼
點擊換一張
注:1.可以使用快捷鍵Alt+S或Ctrl+Enter發(fā)送信息!
2.如有必要,請您留下您的詳細(xì)聯(lián)系方式!

聯(lián)系我們
產(chǎn)品搜索

細(xì)胞系,ATCC細(xì)胞,ATCC菌種,細(xì)胞系目錄,ATCC,鼠傷寒沙門氏菌,atcc細(xì)胞庫,人**瘤細(xì)胞,人食管癌細(xì)胞,ATCC 19404,ATCC 25922,ATCC 19146,微生物菌種,細(xì)胞株,標(biāo)準(zhǔn)菌株,ATCC中國,atcc上海代理,CMCC 菌株,ATCC菌株,細(xì)胞目錄,質(zhì)控菌株,腫瘤細(xì)胞株,乳腺癌細(xì)胞系,人肝癌細(xì)胞系

產(chǎn)品目錄

Copyright? 2003-2025  上海復(fù)祥生物科技有限公司版權(quán)所有            電話:021-35080510
傳真:021-35080520    地址:上海市虹口區(qū)赤峰路630號南雁大廈1901室    郵編: 滬ICP備10013034號-3  

  

滬公網(wǎng)安備 31010902002429號