產(chǎn)品[
Hs 865.Sk 人皮膚成纖維細(xì)胞
]資料
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產(chǎn)品名稱:
Hs 865.Sk 人皮膚成纖維細(xì)胞
產(chǎn)品型號(hào):
CRL-7601
產(chǎn)品廠商:
美國ATCC
產(chǎn)品文檔:
無相關(guān)文檔
簡(jiǎn)單介紹
Hs 865.Sk 人皮膚成纖維細(xì)胞
數(shù)量： 大量
貨號(hào)： xf0331
ATCC? Number: CRL-7601?
Price: $429.00
Designations: Hs 865.Sk
Hs 865.Sk 人皮膚成纖維細(xì)胞
的詳細(xì)介紹
Hs 865.Sk 人皮膚成纖維細(xì)胞
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Biosafety Level:
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1
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frozen
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See Propagation
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adherent
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Homo sapiens (human)
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fibroblast
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Organ: skin
Disease: normal
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In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
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59 years
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female
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Caucasian
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ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
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Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
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culture medium 95%; DMSO, 5%
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Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020
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